1/ Develop a practical nature-based technology

Based on self-purification capacity of constructed wetlands and phage specificity against multidrug-resistant pathogens as a cost-effective and eco-friendly intervention strategy to reduce the risk of dissemination of antimicrobial resistance into the environment.

Work packages 2-6

2/ Evaluate the capacity of constructed wetlands

For the removal of chemical (antibiotic residues) and biological pollutants (antibiotic resistant bacteria (ARB) and antibiotic.

Work packages 3

3/ Select specific phages

Targeting wastewater-derived ARB –focusing on multidrug-resistant ESKAPE pathogens— and to assess their efficacy on the removal of ARB/ARGs from communal wastes.

Work packages 4

4/ Design and scale-up a pilot infrastructure

To implement the proposed PhageLand technology and to assess its efficiency in the removal of antibiotic residues and multidrug-resistant pathogens from well-defined laboratory to complex, real environmental conditions.

Work packages 6

5/ Asses to what extent animals thriving in CW accumulate ARB/ARGs

In their microbiota.

Work packages 3,5

6/ Evaluate the epidemiological risk 
of communal waste

As a source of ARB in relation to public health focusing on Eastern European Low-Middle Income Countries.

Work packages 2,3

7/ Develop a novel method of tracking HGT of ARGs

In various environments by coupling droplet microfluidics and high-throughput fusion PCR amplicon sequencing.

Work packages 3